Intermediates

Reaction: Acetophenone + NADPH = phenylethanol + NADP

Application: Synthesis of enantiomerically pure alcohols with R-configuration

Substrate specificity: Very broad substrate specificity: aliphatic, cyclic and aromatic ketones and diketones

KM VALUE [mM]: 0.08 mM 2,4-pentadion, 0.34 mM 4-Cl-acetophenone at 30癈) and (Km 0.21 mM NADPH at 30癈 and pH 7.5

pH optimum: 6.5 Reduction and 9.0 oxidation

Mr and structure: It is a homotetramer of four identical subunits with a molecular weight of 29.6 kDa

Activity: 1500 U/ml at 30癈 with acetophenone as substrate

Formulation: In glycerol

Stability: Stable at20癈

Commentary: Mg2+ increase the activity

Unit definition: 1 U corresponds to the amount of enzyme which reduces 1祄ol acetophenone per minute at pH 7.0 and 25癈 to phenylethanol